October 18, 2005

• study list

  AHHHHHHH!

  "This is a rough list of topics we
  covered in this section and hence are fair game for exam. Some
  students find this a helpful study aid.

  Study Items for  Exam 2

  DNA double helix structure and base
  pairing

  Nucleotide structure – 3 components

  DNA replication – mechanism, leading
  and lagging strand

  Gene structure, transcription

  Reading genetic code

  Genotype vs phenotype

  Types of mutations – insertion,
  deletion, base change

  Introns vs exons

  Transcription factors – role, types

  Understand lac and trp operons

  pET inducible expression system

  Enhancer elements, activators,
  eukaryotic regulation

  Other ways to regulate protein levels
  besides transcription

  DNA sequencing, dideoxy NTP (describe
  sequencing reaction)

  Steps for sequencing a genome

  Isolating cDNA (describe steps to
  making cDNA library)
  

  Site directed mutagenesis – how do
  you do it
  

  Restriction enzymes and ligating DNA

  Plasmids

  Inducible promotors for expressing
  genes in bacteria

  Homologous recombination

  Making, using, optimizing DNA
  microarrays

  What you learn from microarrays

  Northern blot

  RT PCR – how it works, what you learn

  Molecular beacons

  SNPs – importance, detecting

  PCR to amplify DNA sequence – how it
  works

  Basics of DNA fingerprinting

  mRNA processing

  Gene knockins and knockouts – how to
  you make them
  

  Stem cells

  Tissue specific knock outs

  Ribosome fundamentals

  tRNA structure and function

  Protein translation cycle

  Basic mechanism of protein targeting

  Protein folding and misfolding"

  God, thanks for making such an awesome and complex world... now please help me understand it...... before Thursday night......

  • 10:23 am
  • 3 Comments